Location: Cambridge, MA
Description: Draw on expertise from in vivo based discovery platforms to provide thoughtful guidance on strategies towards candidate profiles for differing target types, including discovery and optimization/ engineering approaches to enhance properties. Design and execute immunization strategies including guidance and definition of best immunogen, adjuvant, and immunization routes, conducting immunizations using transgenic mice, while overseeing strategies with other species such as llama. Execute hands-on wide array of immunization methodologies autonomously, encompassing protein, cell, and DNA materials, utilizing diverse immunization routes and/or gene gun strategies proficiently. Perform animal tissue harvest, B cell separation, and refined enrichment techniques for antigen-specific antibody screening that will include plasma and memory B cell sorting (Beacon and FACS based methods). Perform single-cell RT-PCR, PCR, molecular cloning, and BCR sequence analysis to harvest variable antibody regions for expression and/or repertoire cloning into display platforms, with associated analysis and management of data. Collaborate effectively with high-throughput mammalian expression teams for downstream expression and purification tasks.